Anti-EEF1A1 Polyclonal Antibody (HX255014)

Name: Anti-EEF1A1 Polyclonal Antibody
Brand: abinScience
SKU: HX255014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HX255014

Description

Anti-EEF1A1 Polyclonal Antibody (HX255014) is a rabbit polyclonal antibody detecting EEF1A1 in ELISA, IHC, WB. Suitable for Human, Mouse, Cat, Rat, and Adisura bella.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EEF1A1 (Met1-Pro241).

Target

EEF1A, Elongation factor Tu, Leukocyte receptor cluster member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Eukaryotic elongation factor 1 A-1, EF-Tu, EF1A, eEF1A-1, LENG7, EEF1A1

Purification

Purified by antigen affinity column.

Accession

P68104

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Elongation factor 1-alpha 1 (EEF1A1) is a ~50 kDa protein. Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. Also plays a role in cytoskeleton organization by promoting actin bundling.

1. Ferguson, A. et al. (2015) Molecular cell 60, 475-86. PMID: 26593721

2. Carelli, JD. et al. (2015) eLife 4. PMID: 26651998

3. Wang, HY. et al. (2022) Nature chemistry 14, 1443-1450. PMID: 36123449

4. Juette, MF. et al. (2022) eLife 11. PMID: 36264623

5. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793

6. Maruyama, T. et al. (2007) Clinical and experimental immunology 147, 164-75. PMID: 17177976

Note

For research use only.

Images

References

Datasheet

Anti-EEF1A1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-EEF1A1 Polyclonal Antibody (HX255014)

Overview

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Datasheet

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