Human EEF1A1 Recombinant Protein (N-His) (HX255012)

Name: Human EEF1A1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX255012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX255012
Description	Recombinant Human EEF1A1 Protein, N-His (HX255012) expressed in E. coli, spanning Met1-Pro241. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P68104
Protein length	Met1-Pro241
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.85 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EEF1A, Elongation factor Tu, Leukocyte receptor cluster member 7, Elongation factor 1-alpha 1, EF-1-alpha-1, Eukaryotic elongation factor 1 A-1, EF-Tu, EF1A, eEF1A-1, LENG7, EEF1A1
Background	Elongation factor 1-alpha 1 (EEF1A1) is a ~50 kDa protein. Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. Also plays a role in cytoskeleton organization by promoting actin bundling. 1. Ferguson, A. et al. (2015) Molecular cell 60, 475-86. PMID: 26593721 2. Carelli, JD. et al. (2015) eLife 4. PMID: 26651998 3. Wang, HY. et al. (2022) Nature chemistry 14, 1443-1450. PMID: 36123449 4. Juette, MF. et al. (2022) eLife 11. PMID: 36264623 5. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793 6. Maruyama, T. et al. (2007) Clinical and experimental immunology 147, 164-75. PMID: 17177976
Note	For research use only.

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SDS-PAGE for Recombinant Human EEF1A1 Protein, N-His

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Datasheet

Human EEF1A1 Recombinant Protein (N-His).pdf

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Human EEF1A1 Recombinant Protein (N-His) (HX255012)

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