Anti-EGR1 Polyclonal Antibody (HB800014)

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50ug
100ug

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Overview

Catalog No.

HB800014

Description

Anti-EGR1 Polyclonal Antibody (HB800014) is a rabbit polyclonal antibody detecting EGR1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Cerdocyon thous, Coturnix japonica, and Danio rerio.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Mouse, Bos taurus, Caenorhabditis elegans, Cerdocyon thous, Coturnix japonica, Danio rerio, Chicken, Human, Poecilia reticulata, Rat, Serinus canaria, Taeniopygia guttata, Xenopus laevis, Xenopus tropicalis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EGR1 (Gln282-Ser433).

Target

Zinc finger protein Krox-24, EGR-1, Nerve growth factor-induced protein A, Early growth response protein 1, NGFI-A, KROX24, Transcription factor ETR103, ZNF225, Transcription factor Zif268, Zinc finger protein 225, EGR1, AT225

Purification

Purified by antigen affinity column.

Accession

P18146

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Early growth response protein 1 (EGR1) is a ~57 kDa protein. Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death.

1. Hu, CT. et al. (2010) The FEBS journal 277, 1202-18. PMID: 20121949

2. Hashimoto, H. et al. (2014) Genes & development 28, 2304-13. PMID: 25258363

3. Zandarashvili, L. et al. (2015) FEBS letters 589, 1748-53. PMID: 25999311

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EGR1 polyclonal antibody at 1ug/mL on recombinant EGR1 (Catalog No. HB800012).

References

Datasheet

Anti-EGR1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EGR1 Polyclonal Antibody (HB800014)

Overview

Images

References

Datasheet

SDS

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