Human EGR1 Recombinant Protein (N-His) (HB800012)

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Catalog No.	HB800012
Description	Recombinant Human EGR1 Protein, N-His (HB800012) expressed in E. coli, spanning Gln282-Ser433. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P18146
Protein length	Gln282-Ser433
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.91 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Zinc finger protein Krox-24, EGR-1, Nerve growth factor-induced protein A, Early growth response protein 1, NGFI-A, KROX24, Transcription factor ETR103, ZNF225, Transcription factor Zif268, Zinc finger protein 225, EGR1, AT225
Background	Early growth response protein 1 (EGR1) is a ~57 kDa protein. Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. 1. Hu, CT. et al. (2010) The FEBS journal 277, 1202-18. PMID: 20121949 2. Hashimoto, H. et al. (2014) Genes & development 28, 2304-13. PMID: 25258363 3. Zandarashvili, L. et al. (2015) FEBS letters 589, 1748-53. PMID: 25999311
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human EGR1 Protein, N-His

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HB800012-SDS-PAGE-1.jpg

Datasheet

Human EGR1 Recombinant Protein (N-His).pdf

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Human EGR1 Recombinant Protein (N-His) (HB800012)

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