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Anti-ELOVL3/CIG30 Polyclonal Antibody (MP590014)

Anti-ELOVL3/CIG30 Polyclonal Antibody
Anti-ELOVL3/CIG30 Polyclonal Antibody
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Spec:
  • 50ug
  • 100ug
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Overview
Catalog No.MP590014
Description
Anti-ELOVL3/CIG30 Polyclonal Antibody (MP590014) is a rabbit polyclonal antibody detecting ELOVL3 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse ELOVL3/CIG30 (Asp110-Ser167).
Target 3-keto acyl-CoA synthase ELOVL3, ELOVL FA elongase 3, Elongation of very long chain fatty acids protein 3, CIG30, ELOVL3, Cold-inducible glycoprotein of 30 kDa, Very long chain 3-oxoacyl-CoA synthase 3, ELOVL fatty acid elongase 3, Very long chain 3-ketoacyl-CoA synthase 3
Purification Purified by antigen affinity column.
Accession O35949
RRID Anti-ELOVL3/CIG30 Polyclonal Antibody (abinScience Cat# MP590014, RRID:AB_3730319)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Very long chain fatty acid elongase 3 (ELOVL3/CIG30) is a ~32 kDa protein. Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that exhibits activity toward saturated and unsaturated acyl-CoA substrates with higher activity toward C18 acyl-CoAs, especially C18:0 acyl-CoAs. May participate in the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Participates in the formation of certain VLCFA and triglycerides in certain cells of the hair follicles and the sebaceous glands, required for skin barrier function.

Note For research use only.
Images
  • Anti-ELOVL3/CIG30 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ELOVL3/CIG30 polyclonal antibody at 1ug/mL on recombinant ELOVL3/CIG30 (Catalog No. MP590012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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