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Mouse ELOVL3/CIG30 Recombinant Protein (N-His-SUMO) (MP590012)

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Mouse ELOVL3/CIG30 Recombinant Protein (N-His-SUMO)

Mouse ELOVL3/CIG30 Recombinant Protein (N-His-SUMO)

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Datasheet

Overview

Catalog No.	MP590012
Description	Recombinant Mouse ELOVL3/CIG30 Protein, N-His-SUMO (MP590012) expressed in E. coli. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O35949
Protein length	[Asp110-Ser167]*2
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	3-keto acyl-CoA synthase ELOVL3, ELOVL FA elongase 3, Elongation of very long chain fatty acids protein 3, CIG30, ELOVL3, Cold-inducible glycoprotein of 30 kDa, Very long chain 3-oxoacyl-CoA synthase 3, ELOVL fatty acid elongase 3, Very long chain 3-ketoacyl-CoA synthase 3
Background	Very long chain fatty acid elongase 3 (ELOVL3/CIG30) is a ~32 kDa protein. Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that exhibits activity toward saturated and unsaturated acyl-CoA substrates with higher activity toward C18 acyl-CoAs, especially C18:0 acyl-CoAs. May participate in the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. Participates in the formation of certain VLCFA and triglycerides in certain cells of the hair follicles and the sebaceous glands, required for skin barrier function.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Mouse ELOVL3/CIG30 Protein, N-His-SUMO

References

MP590012-SDS-PAGE-1.jpg

Datasheet

Mouse ELOVL3/CIG30 Recombinant Protein (N-His-SUMO).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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Mouse ELOVL3/CIG30 Recombinant Protein (N-His-SUMO)