Anti-ESR1/ER-alpha Polyclonal Antibody (HY335024)

Name: Anti-ESR1/ER-alpha Polyclonal Antibody
Brand: abinScience
SKU: HY335024
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY335024

Description

Anti-ESR1/ER-alpha Polyclonal Antibody (HY335024) is a rabbit polyclonal antibody detecting ER-alpha in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ESR1/ER-alpha (Met1-Tyr184).

Target

Estrogen receptor, ER-alpha, ESR, ER, ESR1, Nuclear receptor subfamily 3 group A member 1, NR3A1, Estradiol receptor

Purification

Purified by antigen affinity column.

Accession

P03372

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Estrogen receptor (ESR1/ER-alpha) is a ~66 kDa protein. Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner.

1. Molli, PR. et al. (2008) Oncogene 27, 1971-80. PMID: 17922032

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ESR1/ER-alpha polyclonal antibody at 1ug/mL on recombinant ESR1/ER-alpha (Catalog No. HY335022).

References

Datasheet