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Anti-Flag Tag (DYKDDDDK) Recombinant Antibody (M2) (YP666013)

Anti-Flag Tag (DYKDDDDK) Recombinant Antibody (M2)
Anti-Flag Tag (DYKDDDDK) Recombinant Antibody (M2)
Anti-Flag Tag (DYKDDDDK) Recombinant Antibody (M2)
Anti-Flag Tag (DYKDDDDK) Recombinant Antibody (M2)
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Overview
Catalog No.YP666013
Species reactivityGeneral
ApplicationsChIP, ELISA, EIA, FCM, ICC, IF, IP, WB
Host speciesMouse
IsotypeIgG1, kappa
Clone IDM2
Clonality Monoclonal
Target Flag tag, Flag tag (DYKDDDDK-tag), Flag-tag, Flag peptide (DYKDDDDK)
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
ChIP 5-15ug/10^7 cells
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Anti-Flag Tag (DYKDDDDK) Recombinant Antibody (M2)

    SDS-PAGE

    SDS-PAGE for Anti-Flag Tag (DYKDDDDK) Antibody (M2).

  • Anti-Flag Tag (DYKDDDDK) Recombinant Antibody (M2)

    Western blot

    Western blot analysis was performed using anti-Flag tag monoclonal antibody at 1ug/mL on various samples.
    Lane 1: Recombinant protein fused with flag tag
    Lane 2: Recombinant protein fused with flag tag
    Lane 3: Recombinant protein fused with flag tag

Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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