Anti-GABRA5 Recombinant Antibody (N415/24) (HB780013)

Name: Anti-GABRA5 Recombinant Antibody (N415/24)
Brand: abinScience
SKU: HB780013
Price: 196 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB780013

Description

Anti-GABA(A)R, Alpha5 Recombinant Antibody (N415/24) (HB780013) is a mouse monoclonal antibody detecting GABRA5 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N415/24

Target

Gamma-aminobutyric acid receptor subunit alpha-5, GABA(A) receptor subunit alpha-5, GABRA5

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-GABA(A)R, Alpha5 Antibody (N415/24) (abinScience Cat# HB780013, RRID:AB_3728186)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Gamma-aminobutyric acid receptor subunit alpha-5 (GABRA5) is a ~52 kDa protein. Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. GABAARs containing alpha-5/GABRA5 subunits are mainly extrasynaptic and contribute to the tonic GABAergic inhibition in the hippocampus. Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons play a role in learning and memory processes.

1. Caraiscos, VB. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 3662-7. PMID: 14993607

2. Butler, KM. et al. (2018) Brain : a journal of neurology 141, 2392-2405. PMID: 29961870

3. Liu, S. et al. (2018) Cell research 28, 958-961. PMID: 30140029

4. Hernandez, CC. et al. (2019) Brain : a journal of neurology 142, 1938-1954. PMID: 31056671

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-GABA(A)R, Alpha5 Antibody (N415/24)

References

Datasheet

Anti-GABRA5 Recombinant Antibody (N415/24).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GABRA5 Recombinant Antibody (N415/24) (HB780013)

Overview

Images

References

Datasheet

SDS