Anti-GARS1 Polyclonal Antibody (HW710014)

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20ug
50ug
100ug

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Overview
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Overview

Catalog No.

HW710014

Description

Anti-GARS1 Polyclonal Antibody (HW710014) is a rabbit polyclonal antibody detecting GARS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Pongo abelii

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GARS1 (Val567-Lys734).

Target

Diadenosine tetraphosphate synthetase, Glycine--tRNA ligase, GlyRS, Glycyl-tRNA synthetase, GARS1, Glycyl-tRNA synthetase 1, Ap4A synthetase, GARS

Purification

Purified by antigen affinity column.

Accession

P41250

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glycine--tRNA ligase (GARS1) is a ~83 kDa protein. Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.

1. Cader, MZ. et al. (2007) FEBS letters 581, 2959-64. PMID: 17544401

2. Qin, X. et al. (2014) The Journal of biological chemistry 289, 20359-69. PMID: 24898252

3. Oprescu, SN. et al. (2017) Human mutation 38, 1412-1420. PMID: 28675565

4. Guo, RT. et al. (2009) The Journal of biological chemistry 284, 28968-76. PMID: 19710017

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GARS1 polyclonal antibody at 1ug/mL on recombinant GARS1 (Catalog No. HW710012).
Western blot

Western blot analysis was performed using anti-GARS1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: K562 cell lysate
Lane 6: 293T cell lysate
Lane 7: NIH3T3 cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse lung lysate
Lane 10: Rat brain lysate
Lane11: Rat liver lysate
Lane12: Rat lung lysate