Anti-GRP78/HSPA5 Recombinant Antibody (SAA2389) (HY489013)

Name: Anti-GRP78/HSPA5 Recombinant Antibody (SAA2389)
Brand: abinScience
SKU: HY489013
Price: 126 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY489013

Species reactivity

Human

Applications

ELISA, FCM, IF, WB

Host species

Human

Isotype

IgG1(L234A, L235A), kappa

Clone ID

SAA2389

Clonality

Monoclonal

Target

Endoplasmic reticulum chaperone BiP, EC:3.6.4.10, 78 kDa glucose-regulated protein, GRP-78, Binding-immunoglobulin protein, BiP, Heat shock protein 70 family protein 5, HSP70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, HSPA5, GRP78

Endotoxin level

<10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P11021

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Background

Endoplasmic reticulum chaperone BiP (GRP78/HSPA5) is a ~72 kDa protein. Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells.

1. Dana, RC. et al. (1990) Endocrinology 126, 672-4. PMID: 2294010

2. Oka, OB. et al. (2013) Molecular cell 50, 793-804. PMID: 23769672

3. Evensen, NA. et al. (2013) Journal of the National Cancer Institute 105, 1402-16. PMID: 23990668

4. Cuevas, EP. et al. (2017) Scientific reports 7, 44988. PMID: 28332555

5. Ma, K. et al. (2002) The Journal of biological chemistry 277, 18728-35. PMID: 11907036

6. Ng, DT. et al. (1992) Molecular biology of the cell 3, 143-55. PMID: 1550958

7. Oikawa, D. et al. (2009) Experimental cell research 315, 2496-504. PMID: 19538957

8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529

9. Kang, JM. et al. (2015) Cancer research 75, 3087-97. PMID: 26045166

Note

For research use only

Isotype Control

Human IgG1 (LALAPG) Recombinant Isotype Control Antibody (HyHEL-10) (HP688307)
Human IgG1 (LALA) Recombinant Isotype Control Antibody (HyHEL-10) (HP688107)

Images

SDS-PAGE

SDS-PAGE for Anti-GRP78/HSPA5 Antibody (SAA2389)

References

Datasheet

Anti-GRP78/HSPA5 Recombinant Antibody (SAA2389).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GRP78/HSPA5 Recombinant Antibody (SAA2389) (HY489013)

Overview

Images

References

Datasheet

SDS

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