Anti-H2AX Polyclonal Antibody (HB952014) is a rabbit polyclonal antibody detecting H2AX in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Acropora formosa, and Aspergillus niger.
Highlights
â—Affinity Purified — Minimal background and high purity for reliable results.
â—Multi-Application — Validated across multiple applications.
â—Multi-Species — Cross-reactive for translational research.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Histone H2AX is a ~15 kDa protein. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Note
For research use only.
Images
Western blot
Western blot analysis was performed using anti-H2AX polyclonal antibody at 1ug/mL on recombinant H2AX (Catalog No. HB952012).
ä¸æ–‡
English
í•œêµì–´
日本語
Español
Français
РуÑÑкий
