Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody (VK140014)

Name: Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody
Brand: abinScience
SKU: VK140014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

VK140014

Description

Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody (VK140014) is a rabbit polyclonal antibody detecting Minor capsid protein VP1. Suitable for Primate bocaparvovirus 1.

Species reactivity

Primate bocaparvovirus 1 (Human bocavirus 1 type 1) (HBoV1)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HBoV1 Minor capsid protein VP1 (Lys181-Gly290).

Target

EC:3.1.1.4, Minor capsid protein VP1, VP1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q3YPH4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Minor capsid protein VP1 is a ~75 kDa protein. Capsid proteins self-assembles to form an icosahedral capsid with a T=1 symmetry, about 26 nm in diameter, and consisting of 60 copies of three size variants of the capsid proteins, VP1, and VP3, which differ by the presence of an N-terminal extension in the minor protein VP1 (Probable). The capsid has a channel at the 5-fold axis and there are densities extending the 5-fold axis into the interior of the capsid. The capsid encapsulates the genomic ssDNA (Probable). Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region. The additional N-terminal region of isoform Minor capsid protein VP1, called VP1u, may serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.

1. Mietzsch, M. et al. (2017) Journal of virology 91. PMID: 28331084

Note

For research use only

Images

References

Datasheet

Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-HBoV1 Minor capsid protein VP1 Polyclonal Antibody (VK140014)

Overview

Images

References

Datasheet

SDS

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