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Anti-HBV HBsAg Monoclonal Antibody (1A235) (VK539015)

Anti-HBV HBsAg Monoclonal Antibody (1A235)
Anti-HBV HBsAg Monoclonal Antibody (1A235)
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Overview
Catalog No.VK539015
Description
Anti-HBV HBsAg Monoclonal Antibody (1A235) [1A235] (VK539015) is a mouse monoclonal antibody detecting L-HBsAg in ELISA, CLIA, GICA.
Highlights
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHepatitis B virus (HBV)
ApplicationsELISA, CLIA, GICA
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG
Clone ID1A235
Immunogen HBV HBsAg Protein.
Target Large envelope protein, L glycoprotein, L-HBsAg, LHB, Large S protein, Large surface protein, Major surface antigen, S
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Large envelope protein (HBsAg) is a ~42 kDa protein. The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane.

Note For research use only.
Images
  • Anti-HBV HBsAg Monoclonal Antibody (1A235)

    SDS-PAGE

    SDS-PAGE for Anti-HBV HBsAg Monoclonal Antibody (1A235)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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