Anti-Histone H1.2 Recombinant Antibody (N287/65) (HA105023)

Name: Anti-Histone H1.2 Recombinant Antibody (N287/65)
Brand: abinScience
SKU: HA105023
Price: 196 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HA105023

Description

Anti-Histone H1.2 Recombinant Antibody (N287/65) (HA105023) is a mouse monoclonal antibody detecting Histone H1.2 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N287/65

Target

H1-2, H1F2, HIST1H1C, Histone H1.2, Histone H1c, Histone H1d, Histone H1s-1

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-Histone H1.2 Antibody (N287/65) (abinScience Cat# HA105023, RRID:AB_3728646)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Histone H1.2 is a ~21 kDa protein. Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histone H1-2 is required for the condensation of nucleosome chains into higher-order structured fibers. Compared to other histone H1 variants, H1-2 plays an essnetial role in nucleosome condensation: its absence leads to global chromatin decompaction, which is not observed when depleting other histone H1 variants. Histone H1-2 also acts as a histone reader: specifically recognizes and binds histone H3 trimethylated at 'lys-27' (H3K27me3). Histones H1 also promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex, possibly by facilitating restoration of H3K27me3 post-replication.

1. Kim, JM. et al. (2015) Scientific reports 5, 16714. PMID: 26581166

2. Salinas-Pena, M. et al. (2024) eLife 12. PMID: 38530350

3. Liu, C. et al. (2023) Nature communications 14, 4081. PMID: 37429872

4. Matthews, RE. et al. (2025) Molecular cell 85, 2503-2516.e8. PMID: 40516528

5. Pascal, C. et al. (2023) Molecular cell 83, 3801-3817.e8. PMID: 37922872

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Histone H1.2 Antibody (N287/65)

References

Datasheet

Anti-Histone H1.2 Recombinant Antibody (N287/65).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Histone H1.2 Recombinant Antibody (N287/65) (HA105023)

Overview

Images

References

Datasheet

SDS