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Anti-Human alpha-Fetoprotein/AFP Monoclonal Antibody (1A179) (HY276025)

Anti-Human alpha-Fetoprotein/AFP Monoclonal Antibody (1A179)
Anti-Human alpha-Fetoprotein/AFP Monoclonal Antibody (1A179)
Anti-Human alpha-Fetoprotein/AFP Monoclonal Antibody (1A179)
Anti-Human alpha-Fetoprotein/AFP Monoclonal Antibody (1A179)
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Overview
Catalog No.HY276025
Description
Anti-Human AFP/Alpha-fetoprotein Monoclonal Antibody (1A179) [1A179] (HY276025) is a mouse monoclonal antibody detecting Alpha-1-fetoprotein in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Recombinant — Superior batch-to-batch reproducibility with defined sequences.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1
Clone ID1A179
Immunogen E. coli - derived recombinant Human AFP / Alpha-fetoprotein (Leu260-Val609).
Target Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP,
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Alpha-fetoprotein (AFP) is a ~68 kDa protein. Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Note For research use only.
Images
  • Anti-Human alpha-Fetoprotein/AFP Monoclonal Antibody (1A179)

    SDS-PAGE

    SDS-PAGE for Anti-Human AFP/Alpha-fetoprotein Monoclonal Antibody (1A179)

  • Anti-Human alpha-Fetoprotein/AFP Monoclonal Antibody (1A179)

    Immunofluorescence

    Immunofluorescence staining of HepG2 with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cytoplasm.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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