Anti-Human ANO1 Polyclonal Antibody (HP641014)

Name: Anti-Human ANO1 Polyclonal Antibody
Brand: abinScience
SKU: HP641014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP641014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ANO1 (Met117-Gly325).

Target

Anoctamin-1, Tumor-amplified and overexpressed sequence 2, Transmembrane protein 16A, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, TMEM16A, ANO1, TAOS2, ORAOV2, DOG1

Purification

Purified by antigen affinity column.

Accession

Q5XXA6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Anoctamin-1 (ANO1/TMEM16A) is a ~114 kDa protein. Calcium-activated chloride channel (CaCC). Plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression.

1. Schreiber, R. et al. (2010) The Journal of biological chemistry 285, 7838-45. PMID: 20056604

2. Ousingsawat, J. et al. (2011) Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 28, 715-24. PMID: 22178883

3. Tian, Y. et al. (2012) Journal of cell science 125, 4991-8. PMID: 22946059

4. Park, JH. et al. (2021) Journal of medical genetics 58, 247-253. PMID: 32487539

5. Benedetto, R. et al. (2017) Scientific reports 7, 12397. PMID: 28963502

6. Cabrita, I. et al. (2021) American journal of respiratory cell and molecular biology 64, 50-58. PMID: 33026825

7. Simões, FB. et al. (2019) Life science alliance 2. PMID: 31732694

Note

For research use only.

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