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Anti-Human ARFIP2 Polyclonal Antibody (HD593014)

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Overview
Catalog No.HD593014
Description
Anti-Human ARFIP2 Polyclonal Antibody (HD593014) is a rabbit polyclonal antibody detecting ARFIP2. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ARFIP2 (Gly81-Gln341).
Target ADP-ribosylation factor-interacting protein 2, ARFIP2, Arfaptin-2, POR1, Partner of RAC1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P53365
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Arfaptin-2 (ARFIP2) is a ~37 kDa protein. Plays a role in constitutive metalloproteinase (MMP) secretion from the trans Golgi network. May have important functions during vesicle biogenesis at certain cargo subdomains, which could be predominantly utilized by secreted MMPs, such as MMP7 and MMP2. Also involved in autophagy by regulating the starvation-dependent trafficking of ATG9A vesicles which deliver the phosphatidylinositol 4-kinase beta (PI4KB) to the autophagosome initiation site. Involved in phagophore growth during mitophagy by regulating ATG9A trafficking to mitochondria. In addition, plays a role in NF-kappa-B inhibition by interacting with IKBKB and IKBKG.

1. Eiseler, T. et al. (2016) The Journal of biological chemistry 291, 462-77. PMID: 26507660
2. Judith, D. et al. (2019) The Journal of cell biology 218, 1634-1652. PMID: 30917996
3. Judith, D. et al. (2019) Autophagy 15, 1660-1661. PMID: 31204568
4. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106
5. You, DJ. et al. (2015) Cellular signalling 27, 2173-81. PMID: 26296658
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human ARFIP2 Polyclonal Antibody

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