Anti-Human ARL3 Polyclonal Antibody (HW758014)

Name: Anti-Human ARL3 Polyclonal Antibody
Brand: abinScience
SKU: HW758014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
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Overview

Catalog No.

HW758014

Description

Anti-Human ARL3 Polyclonal Antibody (HW758014) is a rabbit polyclonal antibody detecting ARL3 in ELISA, IHC, WB. Suitable for Bovine, Danio rerio, Human, Mouse, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ARL3 (Met1-Lys182).

Target

ARFL3, ADP-ribosylation factor-like protein 3, ARL3

Purification

Purified by antigen affinity column.

Accession

P36405

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ADP-ribosylation factor-like protein 3 (ARL3) is a ~20 kDa protein. Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E. Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium.

1. Zhou, C. et al. (2006) Molecular biology of the cell 17, 2476-87. PMID: 16525022

2. Veltel, S. et al. (2008) FEBS letters 582, 2501-7. PMID: 18588884

3. Wright, KJ. et al. (2011) Genes & development 25, 2347-60. PMID: 22085962

4. Alkanderi, S. et al. (2018) American journal of human genetics 103, 612-620. PMID: 30269812

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ARL3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse heart lysate
Lane 2: Rat heart lysate

References

Datasheet

Anti-Human ARL3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ARL3 Polyclonal Antibody (HW758014)

Overview

Images

References

Datasheet

SDS

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