Human ARL3 Recombinant Protein (N-His) (HW758012)

Name: Human ARL3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW758012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW758012
Description	Recombinant Human ARL3 Protein, N-His (HW758012) expressed in E. coli, spanning Met1-Lys182. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P36405
Protein length	Met1-Lys182
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.62 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ARFL3, ADP-ribosylation factor-like protein 3, ARL3
Background	ADP-ribosylation factor-like protein 3 (ARL3) is a ~20 kDa protein. Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E. Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. 1. Zhou, C. et al. (2006) Molecular biology of the cell 17, 2476-87. PMID: 16525022 2. Veltel, S. et al. (2008) FEBS letters 582, 2501-7. PMID: 18588884 3. Wright, KJ. et al. (2011) Genes & development 25, 2347-60. PMID: 22085962 4. Alkanderi, S. et al. (2018) American journal of human genetics 103, 612-620. PMID: 30269812
Note	For research use only.

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SDS-PAGE for Recombinant Human ARL3 Protein, N-His

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Datasheet

Human ARL3 Recombinant Protein (N-His).pdf

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Human ARL3 Recombinant Protein (N-His) (HW758012)

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