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Anti-Human BCAS2 Polyclonal Antibody (HF603014)

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Overview
Catalog No.HF603014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human BCAS2 (Met1-Phe225).
Target DNA amplified in mammary carcinoma 1 protein, BCAS2, DAM1, Breast carcinoma-amplified sequence 2, Pre-mRNA-splicing factor SPF27, Spliceosome-associated protein SPF 27
Purification Purified by antigen affinity column.
Accession O75934
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Pre-mRNA-splicing factor SPF27 (BCAS2) is a ~26 kDa protein. Required for pre-mRNA splicing as component of the activated spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).

1. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346
2. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770
3. Zhan, X. et al. (2018) Science (New York, N.Y.) 359, 537-545. PMID: 29301961
4. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106
5. Fica, SM. et al. (2019) Science (New York, N.Y.) 363, 710-714. PMID: 30705154
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human BCAS2 Polyclonal Antibody

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