Human BCAS2 Recombinant Protein (N-His) (HF603012)

Name: Human BCAS2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF603012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HF603012
Description	Recombinant Human BCAS2 Protein, N-His (HF603012) expressed in E. coli, spanning Met1-Phe225. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O75934
Protein length	Met1-Phe225
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.29 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNA amplified in mammary carcinoma 1 protein, BCAS2, DAM1, Breast carcinoma-amplified sequence 2, Pre-mRNA-splicing factor SPF27, Spliceosome-associated protein SPF 27
Background	Pre-mRNA-splicing factor SPF27 (BCAS2) is a ~26 kDa protein. Required for pre-mRNA splicing as component of the activated spliceosome. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). 1. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346 2. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770 3. Zhan, X. et al. (2018) Science (New York, N.Y.) 359, 537-545. PMID: 29301961 4. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106 5. Fica, SM. et al. (2019) Science (New York, N.Y.) 363, 710-714. PMID: 30705154
Note	For research use only.

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SDS-PAGE for Recombinant Human BCAS2 Protein, N-His

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Datasheet

Human BCAS2 Recombinant Protein (N-His).pdf

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Human BCAS2 Recombinant Protein (N-His) (HF603012)

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