Anti-Human BEST1 Polyclonal Antibody (HC041014)

Name: Anti-Human BEST1 Polyclonal Antibody
Brand: abinScience
SKU: HC041014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC041014

Description

Anti-Human BEST1 Polyclonal Antibody (HC041014) is a rabbit polyclonal antibody detecting Bestrophin-1; TU15B; Vitelliform macular dystrophy protein 2; BEST1; VMD2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human BEST1 (Arg47-Asp70&Arg200-Asp228&Thr250-Asp270).

Target

Bestrophin-1; TU15B; Vitelliform macular dystrophy protein 2; BEST1; VMD2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O76090

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Bestrophin-1 (BEST1) is a ~67 kDa protein. Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+). Allows the movement of chloride and hydrogencarbonate. Found in a partially open conformation leading to significantly smaller chloride movement. Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation. Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition.

1. Sun, H. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 4008-13. PMID: 11904445

2. Tsunenari, T. et al. (2003) The Journal of biological chemistry 278, 41114-25. PMID: 12907679

3. Burgess, R. et al. (2008) American journal of human genetics 82, 19-31. PMID: 18179881

4. Qu, Z. et al. (2008) American journal of physiology. Cell physiology 294, C1371-7. PMID: 18400985

5. Davidson, AE. et al. (2009) American journal of human genetics 85, 581-92. PMID: 19853238

6. Davidson, AE. et al. (2011) Investigative ophthalmology & visual science 52, 3730-6. PMID: 21330666

7. Johnson, AA. et al. (2015) Investigative ophthalmology & visual science 56, 4619-30. PMID: 26200502

8. Lee, CS. et al. (2015) Investigative ophthalmology & visual science 56, 8141-50. PMID: 26720466

9. Owji, AP. et al. (2022) Nature communications 13, 3836. PMID: 35789156

Note

For research use only

Images

References

Datasheet

Anti-Human BEST1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human BEST1 Polyclonal Antibody (HC041014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us