Human BEST1 Recombinant Protein (N-GST & C-His) (HC041012)

Name: Human BEST1 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HC041012
Price: 211 USD
Availability: InStock

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Catalog No.	HC041012
Description	Recombinant Human BEST1 Protein, N-GST & C-His (HC041012) expressed in E. coli. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O76090
Protein length	Arg47-Asp70&Arg200-Asp228&Thr250-Asp270
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.24 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Bestrophin-1; TU15B; Vitelliform macular dystrophy protein 2; BEST1; VMD2
Background	Bestrophin-1 is a ~67 kDa protein. Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+). Allows the movement of chloride and hydrogencarbonate. Found in a partially open conformation leading to significantly smaller chloride movement. Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation. Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition. 1. Sun, H. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 4008-13. PMID: 11904445 2. Tsunenari, T. et al. (2003) The Journal of biological chemistry 278, 41114-25. PMID: 12907679 3. Burgess, R. et al. (2008) American journal of human genetics 82, 19-31. PMID: 18179881 4. Qu, Z. et al. (2008) American journal of physiology. Cell physiology 294, C1371-7. PMID: 18400985 5. Davidson, AE. et al. (2009) American journal of human genetics 85, 581-92. PMID: 19853238 6. Davidson, AE. et al. (2011) Investigative ophthalmology & visual science 52, 3730-6. PMID: 21330666 7. Johnson, AA. et al. (2015) Investigative ophthalmology & visual science 56, 4619-30. PMID: 26200502 8. Lee, CS. et al. (2015) Investigative ophthalmology & visual science 56, 8141-50. PMID: 26720466 9. Owji, AP. et al. (2022) Nature communications 13, 3836. PMID: 35789156
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human BEST1 Protein, N-GST & C-His

References

Datasheet

Human BEST1 Recombinant Protein (N-GST & C-His).pdf

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Formula

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Human BEST1 Recombinant Protein (N-GST & C-His) (HC041012)

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