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Anti-Human C3aR Polyclonal Antibody (HS803014)

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Overview
Catalog No.HS803014
Description
Anti-Human C3AR1 Polyclonal Antibody (HS803014) is a rabbit polyclonal antibody detecting C3a anaphylatoxin chemotactic receptor; C3AR; C3a-R; C3AR1; AZ3B; C3R1; HNFAG09 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human C3AR1 (Arg161-Arg340).
Target C3a anaphylatoxin chemotactic receptor; C3AR; C3a-R; C3AR1; AZ3B; C3R1; HNFAG09
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession Q16581
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

C3a anaphylatoxin chemotactic receptor (C3AR1) is a ~53 kDa protein. Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a, stimulating chemotaxis, granule enzyme release and superoxide anion production. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. C3AR1 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G alpha proteins and mediates inhibition of adenylate cyclase.

1. Gao, J. et al. (2003) The Journal of biological chemistry 278, 37902-8. PMID: 12871936
2. Yadav, MK. et al. (2023) Cell 186, 4956-4973.e21. PMID: 37852260
3. Ames, RS. et al. (1996) The Journal of biological chemistry 271, 20231-4. PMID: 8702752
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human C3aR Polyclonal Antibody

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