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Anti-Human C3c Polyclonal Antibody (HF636014)

Anti-Human C3c Polyclonal Antibody
Anti-Human C3c Polyclonal Antibody
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Overview
Catalog No.HF636014
Description
Anti-Human C3c Polyclonal Antibody (HF636014) is a rabbit polyclonal antibody detecting Human C3c in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human C3c (Glu1322-Cys1661).
Target C3, ASP, Complement C3, C3adesArg, C3bc, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
Purification Purified by antigen affinity column.
Accession P01024
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Complement C3 (C3c/Complement C3c) is a ~187 kDa protein. Precursor of non-enzymatic components of the classical, alternative, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.

Note For research use only. Not suitable for clinical or therapeutic use.
Images
  • Anti-Human C3c Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Human C3c polyclonal antibody at 1ug/mL on recombinant Human C3c (Catalog No. HF636012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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