Anti-Human CBX5 Polyclonal Antibody (HW777014)

Name: Anti-Human CBX5 Polyclonal Antibody
Brand: abinScience
SKU: HW777014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW777014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CBX5 (Gly2-Lys80).

Target

Heterochromatin protein 1 homolog alpha, Chromobox protein homolog 5, HP1A, Antigen p25, HP1 alpha, CBX5

Purification

Purified by antigen affinity column.

Accession

P45973

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Chromobox protein homolog 5 (CBX5) is a ~22 kDa protein. Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Also recognizes and binds histone H1.4 methylated at 'Lys-26' (H1.4K26me). Excluded from chromatin when histone H1.4 is Simultaneously methylated at Lys-26 (H1.4K26me) and phosphorylated at Ser-27 (H1.4S27Ph). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR).

1. Zhou, L. et al. (2025) Science (New York, N.Y.) 389, 309-316. PMID: 40440427

2. Dawson, MA. et al. (2009) Nature 461, 819-22. PMID: 19783980

3. Daujat, S. et al. (2005) The Journal of biological chemistry 280, 38090-5. PMID: 16127177

4. Kiyomitsu, T. et al. (2010) The Journal of cell biology 188, 791-807. PMID: 20231385

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CBX5 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: K562 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A549 cell lysate