Anti-Human CD104/ITGB4 Recombinant Antibody (65A12) (HB684107)

Name: Anti-Human CD104/ITGB4 Recombinant Antibody (65A12)
Brand: abinScience
SKU: HB684107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HB684107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	65A12
Clonality	Monoclonal
Target	GP150, Integrin beta-4, CD104, ITGB4
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P16144
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Integrin beta-4 (CD104/ITGB4) is a ~202 kDa protein. Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. 1. Ieguchi, K. et al. (2010) The Journal of biological chemistry 285, 31388-98. PMID: 20682778 2. Fujita, M. et al. (2012) The Journal of biological chemistry 287, 12491-500. PMID: 22351760 3. Cedano Prieto, DM. et al. (2017) PloS one 12, e0184285. PMID: 28873464
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human ITGB4 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ITGB4 antibody monoclonal antibody (Catalog # HB684107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human ITGB4 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ITGB4 antibody monoclonal antibody (Catalog # HB684107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human ITGB4 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ITGB4 antibody monoclonal antibody (Catalog # HB684107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

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Formula

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Overview