Anti-Human CD162/SELPLG Recombinant Antibody (SAA2392) (HC298013)

Name: Anti-Human CD162/SELPLG Recombinant Antibody (SAA2392)
Brand: abinScience
SKU: HC298013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC298013

Species reactivity

Human

Applications

ELISA, FCM

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA2392

Clonality

Monoclonal

Target

P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, CD162, SELPLG

Endotoxin level

<10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q14242

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

P-selectin glycoprotein ligand 1 (CD162/SELPLG) is a ~43 kDa protein. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-Human CD162/SELPLG Antibody (SAA2392)
Flow cytometry

Flow-cytometry using APC anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.