Anti-Human CD16a/FCGR3A Recombinant Antibody (SAA2437) (HY386033)

Name: Anti-Human CD16a/FCGR3A Recombinant Antibody (SAA2437)
Brand: abinScience
SKU: HY386033
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY386033

Species reactivity

Human

Applications

ELISA, FACS, Functional assay

Host species

Mouse

Isotype

IgG2a, lambda

Clone ID

SAA2437

Clonality

Monoclonal

Target

Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P08637

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Low affinity immunoglobulin gamma Fc region receptor III-A (CD16a) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation.

1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607

2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335

3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369

4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922

5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175

6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339

7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325

8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432

9. Koene, HR. et al. (1997) Blood 90, 1109-14. PMID: 9242542

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-Human CD16a/FCGR3A Antibody (SAA2437)
Flow cytometry

Flow-cytometry APC using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry PE using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry FITC using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.