Anti-Human CXCR5 Recombinant Antibody (16D7) (HB160107)

Name: Anti-Human CXCR5 Recombinant Antibody (16D7)
Brand: abinScience
SKU: HB160107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HB160107
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	16D7
Clonality	Monoclonal
Target	CXC-R5, CD185, MDR-15, C-X-C chemokine receptor type 5, CXCR5, CXCR-5, MDR15, Monocyte-derived receptor 15, Burkitt lymphoma receptor 1, BLR1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P32302
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	C-X-C chemokine receptor type 5 (CD185/CXCR5) is a ~41 kDa protein. Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD185/CXCR5 Antibody (16D7).
Flow Cytometry

Flow-cytometry using anti-Human CD185/CXCR5 antibody. 293T cells transfected with CD185/CXCR5 (Red Histogram) or Empty vector (Yellow Histogram) with an anti-human CD185/CXCR5 antibody monoclonal antibody (Catalog #HB160107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF647 conjugated Goat anti-Mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD185 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD185 monoclonal antibody (Catalog: HB160107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.