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Anti-Human CD194/CCR4 Recombinant Antibody (SAA0069), PE (HW634127)

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Anti-Human CD194/CCR4 Recombinant Antibody (SAA0069), PE

Anti-Human CD194/CCR4 Recombinant Antibody (SAA0069), PE

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Overview

Catalog No.	HW634127
Description	Anti-Human CD194/CCR4 Antibody (SAA0069), PE [SAA0069] (HW634127) is a mouse monoclonal antibody detecting CD194 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	SAA0069
Conjugation	PE
Clonality	Monoclonal
Target	K5-5, CCR4, C-C CKR-4, CD194, CC-CKR-4, CCR-4, C-C chemokine receptor type 4, CMKBR4
Endotoxin level	Please contact with the lab for this information.
Accession	P51679
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	C-C chemokine receptor type 4 (CD194/CCR4) is a ~41 kDa protein. High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using PE anti-Human CD194 antibody. HDLM-2 cells were stained with an irrelevant antibody (Green Histogram) or a PE anti-Human CD194 antibody monoclonal antibody (Catalog # HW634127 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD194/CCR4 Recombinant Antibody (SAA0069), PE.pdf

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Anti-Human CD194/CCR4 Recombinant Antibody (SAA0069), PE