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Anti-Human CD215/IL15RA Polyclonal Antibody (HC566014)

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Overview
Catalog No.HC566014
Description
Anti-Human CD215/IL15RA Polyclonal Antibody (HC566014) is a rabbit polyclonal antibody detecting IL15RA in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CD215/IL15RA (Cys33-Pro104).
Target Interleukin-15 receptor subunit alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, CD215, Soluble interleukin-15 receptor subunit alpha, sIL-15 receptor subunit alpha, sIL-15R-alpha, sIL-15RA, IL15RA
Purification Purified by antigen affinity column.
Accession Q13261
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Interleukin-15 receptor subunit alpha (CD215/IL15RA) is a ~28 kDa protein. High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. In neutrophils, binds and activates kinase SYK in response to IL15 stimulation. In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner. Expression of different isoforms may alter or interfere with signal transduction.

1. Anderson, DM. et al. (1995) The Journal of biological chemistry 270, 29862-9. PMID: 8530383
2. Ratthé, C. et al. (2004) Journal of leukocyte biology 76, 162-8. PMID: 15123770
3. Dubois, S. et al. (1999) The Journal of biological chemistry 274, 26978-84. PMID: 10480910
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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