Human CD215/IL15RA Recombinant Protein (N-GST & C-His) (HC566012)

Name: Human CD215/IL15RA Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HC566012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC566012
Description	Recombinant Human CD215/IL15RA Protein, N-GST & C-His (HC566012) expressed in E. coli, spanning Cys33-Pro104. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13261
Protein length	Cys33-Pro104
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Interleukin-15 receptor subunit alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, CD215, Soluble interleukin-15 receptor subunit alpha, sIL-15 receptor subunit alpha, sIL-15R-alpha, sIL-15RA, IL15RA
Background	Interleukin-15 receptor subunit alpha (CD215/IL15RA) is a ~28 kDa protein. High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. In neutrophils, binds and activates kinase SYK in response to IL15 stimulation. In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner. Expression of different isoforms may alter or interfere with signal transduction. 1. Anderson, DM. et al. (1995) The Journal of biological chemistry 270, 29862-9. PMID: 8530383 2. Ratthé, C. et al. (2004) Journal of leukocyte biology 76, 162-8. PMID: 15123770 3. Dubois, S. et al. (1999) The Journal of biological chemistry 274, 26978-84. PMID: 10480910
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CD215/IL15RA Protein, N-GST & C-His

References

Datasheet

Human CD215/IL15RA Recombinant Protein (N-GST & C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Human CD215/IL15RA Recombinant Protein (N-GST & C-His) (HC566012)

Overview

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References

Datasheet

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