Anti-Human DNAM-1/CD226 Polyclonal Antibody (HS737014)

Name: Anti-Human DNAM-1/CD226 Polyclonal Antibody
Brand: abinScience
SKU: HS737014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HS737014

Description

Anti-Human CD226/DNAM-1 Polyclonal Antibody (HS737014) is a rabbit polyclonal antibody detecting DNAM-1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD226/DNAM-1 (Glu19-Glu242).

Target

CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, CD226, DNAM1

Purification

Purified by antigen affinity column.

Accession

Q15762

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CD226 antigen (DNAM-1) is a ~38 kDa protein. Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes.

1. Shibuya, A. et al. (1996) Immunity 4, 573-81. PMID: 8673704

2. Shibuya, A. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 161, 1671-6. PMID: 9712030

3. Zhu, Y. et al. (2016) The Journal of experimental medicine 213, 167-76. PMID: 26755705

4. Deuss, FA. et al. (2019) The Journal of biological chemistry 294, 12534-12546. PMID: 31253644

5. Wang, H. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 988-996. PMID: 30591568

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD226 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse spleen lysate
Lane 5: Mouse lung lysate
Lane 6: Rat spleen lysate
Lane 7: Rat lung lysate