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Anti-Human CD235a/GYPA Recombinant Antibody (SAA0500) (HF002023)

Anti-Human CD235a/GYPA Recombinant Antibody (SAA0500)
Anti-Human CD235a/GYPA Recombinant Antibody (SAA0500)
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  • 100ug
  • 1mg
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Overview
Catalog No.HF002023
Description
Anti-Human CD235a/GYPA Recombinant Antibody (SAA0500) (HF002023) is a mouse monoclonal antibody detecting CD235a/GYPA in ELISA, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDSAA0500
Target PAS-2, Glycophorin-A, Sialoglycoprotein alpha, CD235a, MN sialoglycoprotein, GYPA, GPA
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P02724
RRID Anti-Human CD235a/GYPA Antibody (SAA0500) (abinScience Cat# HF002023, RRID:AB_3726021)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Glycophorin-A (CD235a/GYPA) is a ~16 kDa protein. Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane.

1. Vallese, F. et al. (2022) Nature structural & molecular biology 29, 706-718. PMID: 35835865
Note For research use only.
Images
  • Anti-Human CD235a/GYPA Recombinant Antibody (SAA0500)

    SDS-PAGE

    SDS-PAGE for Anti-Human CD235a/GYPA Antibody (SAA0500)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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