Anti-Human CD30/TNFRSF8 Recombinant Antibody (Ber-H2) (HB038307)

Name: Anti-Human CD30/TNFRSF8 Recombinant Antibody (Ber-H2)
Brand: abinScience
SKU: HB038307
Price: 196 USD
Availability: InStock

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Overview

Catalog No.	HB038307
Species reactivity	Human
Applications	ELISA, FCM, IHC, IP, WB
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	Ber-H2
Clonality	Monoclonal
Target	CD30L receptor, Ki-1 antigen, CD30, TNFRSF8, D1S166E, Tumor necrosis factor receptor superfamily member 8, Lymphocyte activation antigen CD30
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P28908
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Tumor necrosis factor receptor superfamily member 8 (CD30/TNFRSF8) is a ~63 kDa protein. Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. CD30 is the therapeutic target of brentuximab vedotin (Adcetris). 1. Smith, CA. et al. (1993) Cell 73, 1349-60. PMID: 8391931 2. Aizawa, S. et al. (1997) The Journal of biological chemistry 272, 2042-5. PMID: 8999898
Note	For research use only.

Images

Bioactivity

Detects CD30/TNFRSF8 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD30 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # HB038307 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.