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Anti-Human CD32b/FCGR2B Recombinant Antibody (6G08), PE (HB103227)

Anti-Human CD32b/FCGR2B Recombinant Antibody (6G08), PE
Anti-Human CD32b/FCGR2B Recombinant Antibody (6G08), PE
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Overview
Catalog No.HB103227
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, lambda
Clone ID6G08
ConjugationPE
Clonality Monoclonal
Target IGFR2, FCG2, Fc-gamma RII-b, CDw32, FcRII-b, IgG Fc receptor II-b, CD32, Fc-gamma-RIIb, Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B
Endotoxin level Please contact with the lab for this information.
Associated products Mouse IgG1 Recombinant Isotype Control Antibody (HyHEL-10), PE
Accession P31994
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Low affinity immunoglobulin gamma Fc region receptor II-b (CD32b/FCGR2B) is a ~34 kDa protein. Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD32b/FCGR2B Recombinant Antibody (6G08), PE

    Flow CytoMetry

    Flow-cytometry using PE anti-human CD32b antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD32b monoclonal antibody (Catalog: HB103227, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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