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Anti-Human CD34 Recombinant Antibody (QBEND-10), APC (HB766237)

Anti-Human CD34 Recombinant Antibody (QBEND-10), APC
Anti-Human CD34 Recombinant Antibody (QBEND-10), APC
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Overview
Catalog No.HB766237
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, lambda
Clone IDQBEND-10
ConjugationAPC
Clonality Monoclonal
Target CD34, Hematopoietic progenitor cell antigen CD34
Endotoxin level Please contact with the lab for this information.
Associated products Mouse IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Accession P28906
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Hematopoietic progenitor cell antigen CD34 is a ~40 kDa protein. Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD34 Recombinant Antibody (QBEND-10), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human CD34 antibody. KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD34 monoclonal antibody (Catalog: HB766237, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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