Anti-Human CD40/TNFRSF5 Recombinant Antibody (SAA2006) (HB782023)

Name: Anti-Human CD40/TNFRSF5 Recombinant Antibody (SAA2006)
Brand: abinScience
SKU: HB782023
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB782023

Species reactivity

Human

Applications

ELISA, WB, FCM

Host species

Human

Isotype

IgG1, kappa

Clone ID

SAA2006

Clonality

Monoclonal

Target

Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, TNFRSF5, CDw40, CD40

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P25942

RRID

Anti-Human CD40/TNFRSF5 Antibody (SAA2006) (abinScience Cat# HB782023, RRID:AB_3727918)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Tumor necrosis factor receptor superfamily member 5 (CD40/TNFRSF5) is a ~30 kDa protein. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

1. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973

Note

For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Human CD40/TNFRSF5 Antibody (ABS2001).
Flow Cytometry

Flow-cytometry using anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD40 monoclonal antibody (Catalog HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD40/TNFRSF5 Antibody (SAA2006).
Flow CytoMetry

Flow-cytometry using APC anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD40 antibody. U2OS cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD40 monoclonal antibody (Catalog: HB782023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.