Anti-Human CD83 Recombinant Antibody (60B10), PE (HX237127)

Name: Anti-Human CD83 Recombinant Antibody (60B10), PE
Brand: abinScience
SKU: HX237127
Price: 214 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.	HX237127
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	60B10
Conjugation	PE
Clonality	Monoclonal
Target	CD83, hCD83, CD83 antigen, B-cell activation protein, Cell surface protein HB15
Endotoxin level	Please contact with the lab for this information.
Associated products	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), PE
Accession	Q01151
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	CD83 antigen is a ~23 kDa protein. Transmembrane glycoprotein predominantly found on the surface of many immune cells including dendritic cells or lymphocytes that plays various roles in immune response regulation. Plays an essential role in CD4(+) T-selection, differentiation and stability by regulating the activity of the major E3 ubiquitin ligase responsible for controlling MHCII trafficking MARCHF8. Also inhibits MARCHF1 association with MHCII or CD86 to prevent their ubiquitination and subsequent degradation. In addition, acts as an important modulator of protective responses against acute infections. 1. Tze, LE. et al. (2011) The Journal of experimental medicine 208, 149-65. PMID: 21220452
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using PE anti-Mouse CD83 antibody. Unstimulated cells (blue Histogram) and 3-day LPS-stimulated Mouse spleen cells (Yellow Histogram) were stained with an FITC anti-Mouse CD83 monoclonal antibody (Catalog: HX237127) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet