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Anti-Human LILRA4/CD85g/ILT7 Polyclonal Antibody (HX894014)

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Overview
Catalog No.HX894014
Description
Anti-Human CD85g/LILRA4/ILT7 Polyclonal Antibody (HX894014) is a rabbit polyclonal antibody detecting LILRA4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CD85g/LILRA4/ILT7 (Glu24-Gly419).
Target Leukocyte immunoglobulin-like receptor subfamily A member 4, CD85 antigen-like family member G, Immunoglobulin-like transcript 7, ILT-7, CD85g, LILRA4, ILT7
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P59901
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Leukocyte immunoglobulin-like receptor subfamily A member 4 (CD85g/LILRA4) is a ~55 kDa protein. Functions coreceptor to limit the innate immune responses to viral infections; signaling occurs via FCER1G. Down-regulates the production of IFNA1, IFNA2, IFNA4, IFNB1 and TNF by plasmacytoid dendritic cells that have been exposed to influenza virus or cytidine-phosphate-guanosine (CpG) dinucleotides, indicating it functions as a negative regulator of TLR7 and TLR9 signaling cascades. Down-regulates interferon production in response to interaction with BST2 on HIV-1 infected cells. Activates a signaling cascade in complex with FCER1G that results in phosphorylation of Src family and Syk kinases and thereby triggers mobilization of intracellular Ca(2+). Does not interfere with the differentiation of plasmacytoid dendritic cells into antigen-presenting cells.

1. Cao, W. et al. (2006) The Journal of experimental medicine 203, 1399-405. PMID: 16735691
2. Cao, W. et al. (2009) The Journal of experimental medicine 206, 1603-14. PMID: 19564354
3. Tavano, B. et al. (2014) PloS one 9, e89414. PMID: 24586760
4. Bego, MG. et al. (2015) PLoS pathogens 11, e1005024. PMID: 26172439
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human LILRA4/CD85g/ILT7 Polyclonal Antibody

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