Anti-Human CEP131 Polyclonal Antibody (HP795014)

Name: Anti-Human CEP131 Polyclonal Antibody
Brand: abinScience
SKU: HP795014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP795014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CEP131 (Arg750-Ala1020).

Target

AZI1, Pre-acrosome localization protein 1, Centrosomal protein of 131 kDa, KIAA1118, CEP131, 5-azacytidine-induced protein 1

Purification

Purified by antigen affinity column.

Accession

Q9UPN4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Centrosomal protein of 131 kDa (CEP131) is a ~122 kDa protein. Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation. In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner. Also acts as a negative regulator of BBSome ciliary trafficking. Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail.

1. Graser, S. et al. (2007) The Journal of cell biology 179, 321-30. PMID: 17954613

2. Chavali, PL. et al. (2013) The EMBO journal 32, 3011-3. PMID: 24185901

3. Villumsen, BH. et al. (2013) The EMBO journal 32, 3029-40. PMID: 24121310

4. Tollenaere, MAX. et al. (2015) Nature communications 6, 10075. PMID: 26616734

5. Chamling, X. et al. (2014) PLoS genetics 10, e1004083. PMID: 24550735

6. Staples, CJ. et al. (2012) Journal of cell science 125, 4770-9. PMID: 22797915

7. Kodani, A. et al. (2015) eLife 4. PMID: 26297806

8. Denu, RA. et al. (2019) The Journal of biological chemistry 294, 6531-6549. PMID: 30804208

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CEP131 polyclonal antibody at 1µg/ml on various samples.
Lane 1: Mouse testis lysate
Lane 2: Rat testis lysate

References

Datasheet

Anti-Human CEP131 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human CEP131 Polyclonal Antibody (HP795014)

Overview

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References

Datasheet

SDS

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