Human CEP131 Recombinant Protein (N-His) (HP795012)

Name: Human CEP131 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP795012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP795012
Description	Recombinant Human CEP131 Protein, N-His (HP795012) expressed in E. coli, spanning Arg750-Ala1020. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9UPN4
Protein length	Arg750-Ala1020
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.68 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	AZI1, Pre-acrosome localization protein 1, Centrosomal protein of 131 kDa, KIAA1118, CEP131, 5-azacytidine-induced protein 1
Background	Centrosomal protein of 131 kDa (CEP131) is a ~122 kDa protein. Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation. In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner. Also acts as a negative regulator of BBSome ciliary trafficking. Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail. 1. Graser, S. et al. (2007) The Journal of cell biology 179, 321-30. PMID: 17954613 2. Chavali, PL. et al. (2013) The EMBO journal 32, 3011-3. PMID: 24185901 3. Villumsen, BH. et al. (2013) The EMBO journal 32, 3029-40. PMID: 24121310 4. Tollenaere, MAX. et al. (2015) Nature communications 6, 10075. PMID: 26616734 5. Chamling, X. et al. (2014) PLoS genetics 10, e1004083. PMID: 24550735 6. Staples, CJ. et al. (2012) Journal of cell science 125, 4770-9. PMID: 22797915 7. Kodani, A. et al. (2015) eLife 4. PMID: 26297806 8. Denu, RA. et al. (2019) The Journal of biological chemistry 294, 6531-6549. PMID: 30804208
Note	For research use only.

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SDS-PAGE for Recombinant Human CEP131 Protein, N-His

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Datasheet

Human CEP131 Recombinant Protein (N-His).pdf

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Human CEP131 Recombinant Protein (N-His) (HP795012)

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