Anti-Human CIAO1 Polyclonal Antibody (HF508014)

Name: Anti-Human CIAO1 Polyclonal Antibody
Brand: abinScience
SKU: HF508014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF508014

Description

Anti-Human CIAO1 Polyclonal Antibody (HF508014) is a rabbit polyclonal antibody detecting CIAO1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CIAO1 (Met1-Leu339).

Target

Probable cytosolic iron-sulfur protein assembly protein CIAO1, WD repeat-containing protein 39, CIAO1, CIA1, WDR39

Purification

Purified by antigen affinity column.

Accession

O76071

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Probable cytosolic iron-sulfur protein assembly protein CIAO1 is a ~37 kDa protein. Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2. Seems to specifically modulate the transactivation activity of WT1.

1. Srinivasan, V. et al. (2007) Structure (London, England : 1993) 15, 1246-57. PMID: 17937914

2. Stehling, O. et al. (2013) Cell metabolism 18, 187-98. PMID: 23891004

3. Maio, N. et al. (2024) The Journal of clinical investigation 134. PMID: 38950322

4. Johnstone, RW. et al. (1998) The Journal of biological chemistry 273, 10880-7. PMID: 9556563

5. Ito, S. et al. (2010) Molecular cell 39, 632-40. PMID: 20797633

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CIAO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: PC-3 cell lysate