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Anti-Human CP Polyclonal Antibody (HF643014)

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Overview
Catalog No.HF643014
Description
Anti-Human CP Polyclonal Antibody (HF643014) is a rabbit polyclonal antibody detecting Ceruloplasmin; 1.16.3.1; Ferroxidase; CP. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CP (Asp789-Gly1065).
Target Ceruloplasmin; 1.16.3.1; Ferroxidase; CP
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P00450
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Ceruloplasmin (CP) is a ~122 kDa protein. Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin.

1. Hochstrasser, H. et al. (2005) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 19, 1851-3. PMID: 16150804
2. Stoj, C. et al. (2003) FEBS letters 554, 422-6. PMID: 14623105
3. Young, SN. et al. (1972) The Biochemical journal 129, 273-83. PMID: 4643313
4. Osaki, S. et al. (1966) The Journal of biological chemistry 241, 2746-51. PMID: 5912351
5. Park, YS. et al. (1999) FEBS letters 458, 133-6. PMID: 10481051
6. Shiva, S. et al. (2006) Nature chemical biology 2, 486-93. PMID: 16906150
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CP Polyclonal Antibody

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