Human CP Recombinant Protein (N-His) (HF643012)

Name: Human CP Recombinant Protein (N-His)
Brand: abinScience
SKU: HF643012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HF643012
Description	Recombinant Human CP Protein, N-His (HF643012) expressed in E. coli, spanning Asp789-Gly1065. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P00450
Protein length	Asp789-Gly1065
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.00 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CP, Ceruloplasmin, Ferroxidase
Background	Ceruloplasmin (CP) is a ~122 kDa protein. Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin. 1. Hochstrasser, H. et al. (2005) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 19, 1851-3. PMID: 16150804 2. Stoj, C. et al. (2003) FEBS letters 554, 422-6. PMID: 14623105 3. Young, SN. et al. (1972) The Biochemical journal 129, 273-83. PMID: 4643313 4. Osaki, S. et al. (1966) The Journal of biological chemistry 241, 2746-51. PMID: 5912351 5. Park, YS. et al. (1999) FEBS letters 458, 133-6. PMID: 10481051 6. Shiva, S. et al. (2006) Nature chemical biology 2, 486-93. PMID: 16906150
Note	For research use only.

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SDS-PAGE for Recombinant Human CP Protein, N-His

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Datasheet

Human CP Recombinant Protein (N-His).pdf

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C₁ × V₁ = C₂ × V₂

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Human CP Recombinant Protein (N-His) (HF643012)

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