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Anti-Human CX3CL1/Fractalkine Polyclonal Antibody (HX143014)

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Overview
Catalog No.HX143014
Description
Anti-Human CX3CL1/Fractalkine Polyclonal Antibody (HX143014) is a rabbit polyclonal antibody detecting Fractalkine in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CX3CL1/Fractalkine (Asp90-Gln245).
Target Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, Processed fractalkine, CX3CL1, FKN, NTT, SCYD1
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P78423
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Fractalkine (CX3CL1) is a ~42 kDa protein. Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins.

1. Nishimura, M. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 6173-80. PMID: 12055230
2. Xue, X. et al. (2011) PLoS pathogens 7, e1002162. PMID: 21829356
3. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415
4. Fong, AM. et al. (1998) The Journal of experimental medicine 188, 1413-9. PMID: 9782118
5. Mizoue, LS. et al. (1999) Biochemistry 38, 1402-14. PMID: 9931005
6. Bazan, JF. et al. (1997) Nature 385, 640-4. PMID: 9024663
7. Pan, Y. et al. (1997) Nature 387, 611-7. PMID: 9177350
8. Fujita, M. et al. (2014) PloS one 9, e96372. PMID: 24789099
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CX3CL1/Fractalkine Polyclonal Antibody

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