Anti-Human CYP1B1 Polyclonal Antibody (HA565014)

Name: Anti-Human CYP1B1 Polyclonal Antibody
Brand: abinScience
SKU: HA565014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HA565014

Description

Anti-Human CYP1B1 Polyclonal Antibody (HA565014) is a rabbit polyclonal antibody detecting CYP1B1. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CYP1B1 (Leu224-Asp492).

Target

CYP1B1, CYPIB1, Cytochrome P450 1B1, EC:1.14.14.1, EC:4.2.1.152, Hydroperoxy icosatetraenoate dehydratase

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q16678

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Cytochrome P450 1B1 (CYP1B1) is a ~60 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position. Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites.

1. Chen, H. et al. (2000) Drug metabolism and disposition: the biological fate of chemicals 28, 315-22. PMID: 10681376

2. Badawi, AF. et al. (2001) Metabolism: clinical and experimental 50, 1001-3. PMID: 11555828

3. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317

4. Choudhary, D. et al. (2004) Drug metabolism and disposition: the biological fate of chemicals 32, 840-7. PMID: 15258110

5. Mesaros, C. et al. (2010) Rapid communications in mass spectrometry : RCM 24, 3237-47. PMID: 20972997

Note

For research use only

Images

Western blot

Lane 1: Hela cell lysate

References

Datasheet

Anti-Human CYP1B1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CYP1B1 Polyclonal Antibody (HA565014)

Overview

Images

References

Datasheet

SDS

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