Human CYP1B1 Recombinant Protein (N-GST) (HA565012)

Name: Human CYP1B1 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HA565012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA565012
Description	Recombinant Human CYP1B1 Protein, N-GST (HA565012) expressed in E. coli, spanning Leu224-Asp492. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q16678
Protein length	Leu224-Asp492
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	57.10 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Cytochrome P450 1B1, EC:1.14.14.1, CYPIB1, Hydroperoxy icosatetraenoate dehydratase, EC:4.2.1.152, CYP1B1
Background	Cytochrome P450 1B1 (CYP1B1) is a ~60 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position. Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites. 1. Chen, H. et al. (2000) Drug metabolism and disposition: the biological fate of chemicals 28, 315-22. PMID: 10681376 2. Badawi, AF. et al. (2001) Metabolism: clinical and experimental 50, 1001-3. PMID: 11555828 3. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317 4. Choudhary, D. et al. (2004) Drug metabolism and disposition: the biological fate of chemicals 32, 840-7. PMID: 15258110 5. Mesaros, C. et al. (2010) Rapid communications in mass spectrometry : RCM 24, 3237-47. PMID: 20972997
Note	For research use only.

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SDS-PAGE for Recombinant Human CYP1B1 Protein, N-GST

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Datasheet

Human CYP1B1 Recombinant Protein (N-GST).pdf

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Human CYP1B1 Recombinant Protein (N-GST) (HA565012)

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